Dr.Maisch HPLC GmbH
Beim Brückle 14,
72119 Ammerbuch

Tel.: (+49)7073 50357   Fax: (+49)7073 4216 

E-Mail: maisch@reprosil.com

nternet: reprosil.com

Chiralic Analysis

UltraSep ES CHIR P

(Pirkle phases)

In UltraSep ES CHIR P (P means Pirkle) 3,5-DNB-D-a-phenylglycine is coupled to the gel matrix via a spacer by an amide type bond (CHIRP, D-Phegly). The 3,5-dinitrobenzoyl (DNB) residue acts as a p-electron acceptor yielding good separation conditions for enantiomers with p-electrons. For some separations the analogously constructed DNB-L-leucine phase with a different selectivity is of advantage (CHIRP, L-Leu). This shows the peak reversal of the (+) and (-) forms of 2,2,2-trifluoro-1-(9-anthryl)ethanol on both packings (Application CHIRP).

UltraSep ES CHIR PMA

(Polymer phase)

This packing has a layer of polymeric methacrylic acid (PMA) on silica. It is characterized by hydrophobic properties. Application CHIRPMA shows the separation of 2,2,2-trifluoro-1-(9-anthryl)ethanol, which is often used as "chiralic probe". As a practical example the separation of the optical isomers of the diuretic Cyclopenthiazid (Navidrex) is also demonstrated..

UltraSep ES RP Hypro Cu

(Ligand exchanger)

UltraSep ES RP Hypro Cu was developed especially for the chiralic separation of amino acids and derivatized amino acids.

Underivatized amino acids can be separated into their enantiomers by ligand exchange chromatography. The special problem was besides, to determine the N-acetyl compounds. All commercial ligand exchangers as far as checked were not able to do this.

For this reason SEPSERV developed  a permanently loaded ligand exchange carrier on basis of RP . The combination of two chromatographical effects made it possible to achieve the wanted separation (Application CHIRLIG).

Chiralic Phases on the Basis of Carbohydrates

Applications:  Pharmaceutical active compounds
Chiralic compounds in foods, dyes and preservatives
Chiralic catalysts and bioracemates
The Eurocel phases are comparable with the corresponding Chiracel types.

 

Phase Polysaccharide Modification Application Examples

Eurocel 01

corresp. Chiralcel OD

Cellulose 3,5-Dimethylphenyl-carbamate Alkaloides, tropines, amines, beta blockers

Eurocel 02

corresp. Chiralcel OB

Cellulose Benzoate Small aliphatic and aromatic compounds

Eurocel 03

corresp. Chiralcel OJ

Cellulose 4-Methylbenzoate Arylmethyl esters, Arylmethoxy esters

Eurocel 04

corresp. Chiralcel OC

Cellulose Phenylcarbamate Cyclopentenones
The phases exhibit a very good stability, a high capacity as well as a great flexibilty relating to eluents. This is demonstrated by the example PROPRANOLOL.
Name us your separation problem or order the list of applications via e-mail.
Particle sizes: 3, 5, 10, and 20 m. Column diameters: 2, 4, 4.6, 8, 16, and 20 mm. Column lengths: 125, 150, and 250 mm (analytical columns) and 30, 120, and 250 mm (preparative columns).

 

Examples of Separated Optical Antipodes

Analyzed in Application

Go to

D,L-Acetylmethionine

CHIRLIG

CHIRP

Cyclopenthiazid (Navidrex)

CHIRPMA

D,L-Methionine

CHIRLIG

CHIRPMA

(+,-)2,2,2-Trifluoro-1-(9-anthryl)ethanol

CHIRP, CHIRPMA

CHIRLIG

 

COLUMNS and  PRICES  ()
  Column size (mm)
250 x 4 250 x 3 250 x 2 125 x 3 125 x 2
UltraSep ES CHIR P,D-Phegly       410.00 390.00
UltraSep ES CHIR P,L-Leu   530.00 465.00    
UltraSep ES CHIR PMA   620.00 590.00 560.00 495.00
UltraSep ES RP Hypro Cu 345.00 325.00 325.00    
Eurocel 01

Columns and Prices for Eurocel 01

Eurocel 02 Columns and Prices for Eurocel 02
Eurocel 03 Columns and Prices for Eurocel 03
Eurocel 04 Columns and Prices for Eurocel 04
 

Size (mm)

 
10 x 4 10 x 3 10 x 2
Precolumn cartridges for RP Hypro Cu
(Price per pack = 5 pieces)
210.00 195.00 195.00
 

 

 

 

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