|
Application
AA |
|
|
Packing:
|
UltraSep
ESD AP
|
|
Column:
|
250
x 3 mm
|
|
Detection:
|
UV
436 nm
|
|
Eluent:
|
A:
Acetate buffer pH 6.5
B: Acetonitrile/isopropanol (2/3)
|
|
Gradient:
|
Start
4% B
60th min 100% B |
|
Flow
rate:
|
0.6
ml/min
|
|
Temperature:
|
45
°C
|
|
Sample
diluted 1:1 with acetate buffer
|
|
1 |
Aspartic acid (Asp) |
11 |
Methionine (Met) |
|
2 |
Glutamic acid (Glu) |
12 |
Isoleucine (Ile) |
|
3 |
DABSOH |
13 |
Leucine (Leu) |
|
|
(Methylorange) |
14 |
Phenylalanine (Phe) |
|
4 |
Serine (Ser) |
15 |
unknown |
| 5 |
Threonine (Thr) |
16 |
Cystine (Cys2) |
| 6 |
Glycine (Gly) |
17 |
Ornithine (Orn) |
| 7 |
Alanine (Ala) |
18 |
Lysine (Lys) |
| 8 |
Arginine (Arg) |
19 |
Histidine (His) |
| 9 |
Proline (Pro) |
20 |
Tyrosine (Tyr) |
| 10 |
Valine (Val) |
|
|
| Amino acids as DABSYL derivatives |
|
|
|
|
|
| |
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