|
|
Packing:
|
UltraSep
ES DIISO
|
|
Column:
|
250
x 4 mm
|
|
Detection:
|
UV
254 nm
|
|
Eluent:
|
A: 0.1 M ammonia acetate pH 6.2
(glacial acetic acid)/2% acetonitrile
B: Acetonitrile
|
|
Gradient:
|
Start
27.3% B
17th min 27.3% B
18th min 40% B
30th min 40% B
40th min 27.3. min |
|
Flow
:
|
1.65 ml/min
|
|
Temperature:
|
25
°C
|
| Derivatized
with: |
1-(2-pyridyl)piperazine |
|
1 |
2,6-Toluylenediisocyanate
(2,6-TDI) |
|
2 |
Hexamethylene-1,6-diisocyanate
(HDI) |
|
3 |
2,4-Toluylenediisocyanate
(2,4-TDI) |
| 4 |
cis-Isophorondiisocyanate
(cis-IPDI) |
| 5 |
trans-Isophorondiisocyanate
(trans-IPDI) |
|
6 |
4,4’-Methylenediphenylisocyanate (MDI) |
|
Chromatogram of a customer: Wehrwissenschaftliches Institut Munster
|