SEPSERV
HPLC

Separation Service Berlin
Helmholtzstr. 2 - 9, Aufg. L, D-10587 Berlin
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Tel.:  (030) 393 09 91    Fax: (030) 393 49 25  oder  391 01 000

E-Mail: sepserv.berlin@t-online.de  Internet: www.sepserv.de

Fruit Acids and Fatty Acids

UltraSep ESD FS

For the separation of small molecular acids, especially of fruit acids in food stuffs and similar materials, the packing UltraSep ES FS was developed. In the simplest case it is possible to use the method of ion suppression (Application FS1). If the mixtures of fruit acids cannot be separated satisfactory in this manner, individual solutions can be achieved by ion pairing chromatography (IPC; Application FS2). 

UltraSep ES WAX FS and UltraSep ES WAXI FS

For the case that special mixtures of mono- and di- or tricarbonic acids cannot be separated satisfactory by the methodical variants mentioned above, it is possible to determine the monocarbonic acids separately by the ion exchanger UltraSep ES WAX FS (Application FS3). Di- and tricarbonic acids will be removed from the column by back-flushing and, if necessary, separated by UltraSep ESD FS. 

The weak imino groups carrying ion exchanger UltraSep ES WAXI FS extends the selectivity of UltraSep ES WAX FS for monocarbonic acids within the pH range of 3.0 3.5 (Application FS4).

UltraSep ES FS

With this packing the separation of homologeous fatty acids by gradient elution is no problem (Application FS5). For the higher fatty acids we recommend UltraSep RP18 M500 (Application FS6).

 

 

Examples of Analyzed Compounds

Analyzed in Application

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Acetic acid

FS1, FS3, FS4, FS5

Arachinic acid

FS6

iso-Butyric acid

FS5

FS1

n-Butyric acid

FS5

Caprinic acid 

FS6

iso-Capronic acid

FS5

FS2

n-Capronic acid

FS5

Citric acid

FS1, FS2

FS3

Elaidinic acid

FS6

Formic acid

FS1, FS2, FS3, FS4

Fumaric acid

FS1, FS2

FS4

Glycolic acid

FS2, FS3, FS4

Lactic acid

FS1, FS3, FS4

FS5

Lauric acid

FS6

Malic acid

FS1

FS6

Myristic acid

FS6

Oxalic acid

FS2

Palmitinic acid

FS6

Propionic acid

FS5

Quinic acid

FS2, FS3, FS4

Shikimic acid

FS4

Stearic acid

FS6

Succinic acid

FS1, FS2

Tartaric acid

FS1, FS2

iso-Valeric acid

FS5

n-Valeric acid

FS5

Vitamin C

FS3, FS4

Separation of carboxylic acids with Polymeric Phases POL

 

COLUMNS and  PRICES  ()
  Column size (mm)
250 x 4 250 x 3 250 x 2 125 x 4 125 x 3 125 x 2
UltraSep ESD FS 360.00 340.00 355.00 320.00 295.00 290.00
UltraSep ES WAX FS 250.00 225.00 245.00 220.00 195.00 215.00
UltraSep ES WAX FSI 290.00 290.00 290.00 265.00 265.00 265.00
UltraSep ES FS 220.00 220.00 240.00 195.00 195.00 205.00
 

Size (mm)

  
10 x 4 10 x 3 10 x 2
Precolumn cartridges
(Price per pack = 5 pieces)
170.00 170.00 170.00
 

 

 

 

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